Vector Synthesis Research Associate II
Vertex Pharmaceuticals Inc (US)

Boston, Massachusetts

Posted in Retail
about 1 month ago

This job has expired.

Job Info

In June 2019, Vertex announced the establishment of a new research site in the Boston area where research, development, and clinical manufacturing for cell and genetic therapies will be primarily based. Cell and genetic therapies represent two rapidly emerging therapeutic modalities with the potential to treat-and even cure-several of the diseases Vertex is focused on, including sickle cell disease, Duchenne muscular dystrophy and type 1 diabetes. Further investment in a research site specifically designed to advance these programs will be key to Vertex's continued and sustained leadership in these exciting approaches. At Vertex Cell and Genetic Therapies (VCGT) our research teams will bring together the best biology, technologies and enhanced manufacturing capabilities to ensure we bring these cutting-edge transformative therapies to patients as quickly as possible.

Key responsibilities

  • Responsible to execute protocols in general molecular biology techniques, including PCR, DNA purification, Gel electrophoresis, conventional restriction enzyme digestion & ligation, DNA sequencing

  • Run experiments in recombinant DNA assembly methodologies, including Gibson Assembly, PCR Assembly, and facilitated recombination (i.e., Gateway cloning) methodologies; protein expression and purification, including HPLC, western blot, affinity resin purification

  • Experience producing and purifying plasmid DNAs with a wide variety of genetic elements and inherent yield

  • Operate and maintain laboratory equipment

  • Maintain clear and complete experimental records

  • Collaborate with a research team to facilitate the timely execution of projects

  • Additional duties and responsibilities as required

Preferred Qualifications:

  • Bachelor's degree in molecular biology, and 1-4 years of relevant experience

  • Experience with production of bacterial plasmids

  • Excellent organizational skills and documentation practices

  • Strong attention to detail

  • Excellent communication skills; verbal and written

  • Ability to work both independently and as part of a team

  • Ability to ask for help if next steps are unclear

  • Hands-on experience with molecular biology techniques

This job has expired.

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